FIG 5.

Effect of TFV on 5′-nucleotidase gene expression in CD14⁺ cells. CD14⁺ cells were isolated from Em and treated with 1 mg/ml of TFV for 24 h. RNA was isolated, and RT- PCR was performed to compare the levels of mRNA of the nucleotidase genes NT5E, NT5C2, NT5C3L, NT5C1A, NT5C, and NT5M. Six to 9 independent experiments with different patients were done. *, P < 0.05. Relative mRNA expression (A) and fold change in the mRNA expression (B) of NT5E, NT5C2, NT5C3L, NT5C1A, NT5C, and NT5M gene expression in the presence of TFV from CD14⁺ cells in Em. Each TFV-treated sample was compared with its own control, and fold changes in mRNA expression were calculated by converting each control to 1.