FIG 6.

Effect of TFV on 5′-nucleotidase activity in epithelial cells, fibroblasts, CD4⁺ T cells, and CD14⁺ cells. 5′-nucleotidase biological activity was measured (see Materials and Methods) in epithelial cells, fibroblasts, and CD4⁺ T cells from Em, Cx, and Ecx and from CD14⁺ cells from Em after 24 h with 1 mg/ml of TFV treatment. Values are expressed as milli-enzyme units (mEU) per 1 million cells. Bars represent means ± SEM from 5 or 6 separate experiments with different donors. *, P < 0.05. Epithelial cells (A) fibroblasts (B), CD4⁺ T cells (C), and CD14⁺ cells (D) from Em, Cx, and Ecx after 24 h with 1 mg/ml of TFV treatment.