TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Organism and strain | ||
| E. coli | ||
| DH5α | Host strain for plasmids | Lab stock |
| WM3064 | Donor strain for conjugation; ΔdapA | W. Metcalf, UIUCa |
| P. aeruginosa | ||
| PAO1 | Reference strain | 36 |
| PAO1ΔDDh2Dh3 | PAO1 with the three ampD homologues deleted | 36 |
| PAO1ΔG | PAO1 with ampG (PA4393) deleted | 37 |
| S. oneidensis | ||
| MR-1 | Wild type | Lab stock |
| HG0421 | ΔampD derived from MR-1 | This study |
| HG0422 | ΔampE derived from MR-1 | This study |
| HG0837 | ΔblaA derived from MR-1 | 26 |
| HG2250 | ΔnagZ derived from MR-1 | This study |
| HG3814 | ΔampG derived from MR-1 | This study |
| HG2250–0837 | ΔnagZΔblaA derived from MR-1 | This study |
| HG3814–0837 | ΔampGΔblaA derived from MR-1 | This study |
| HG0421–2250 | ΔampDΔnagZ derived from MR-1 | This study |
| Plasmids | ||
| pHGM01 | Apr Gmr Cmr suicide vector | 29 |
| pHG101 | Promoterless vector for complementation | 30 |
| pHGEI01 | Integrative lacZ reporter vector | 31 |
| pTP327-PblaA | pTP327 containing the blaA promoter | 26 |
| pHGEI01-PblaA | pHGI01 containing the blaA promoter | This study |
a
UIUC, University of Illinois at Urbana-Champaign.