TABLE 4.
Effects of CCCP (1 μM) on ampicillin resistance and BlaA expression in S. oneidensis wild type and its derivate strains
| Strain or mutationa | MIC (μg/ml) | β-Lactamase activity (mean ± SD) withb: |
|
|---|---|---|---|
| No inducer | Inducer (200 μg/ml) | ||
| WT | 16 | 7 ± 1.3 | 69 ± 6.4 |
| ΔampG | 128 | 12 ± 2.7 | 178 ± 23 |
| ΔampG/ampG | 16 | 8 ± 1.6 | 58 ± 7 |
a
ΔampG/ampG represents the ΔampG strain that was complemented with S. oneidensis genes in trans.
b
Nanomoles of nitrocefin hydrolyzed per minute per milligram of protein. Induction was carried out with ampicillin for 2 h.