TABLE 3.
Prevalence of virulence genotype subclone according to clonal subset among 128 archival ST131 Escherichia coli isolates (1968 to 2011)
| Virulence category | Specific virulence trait(s)b,c,d | No. (column %) of isolates with indicated trait |
P valuea |
|||||
|---|---|---|---|---|---|---|---|---|
| Total (n = 128) | Non-H30 (n = 62) | H30 (non-Rx) (n = 17) | H30-Rx (n = 49) | Non-H30 vs H30 (non-Rx) | Non-H30 vs H30-Rx | H30 (non-Rx) vs H30-Rx | ||
| Adhesins | afa and dra | 56 (44) | 19 (31) | 0 (0) | 37 (76) | 0.008 | <0.001 | <0.001 |
| iha | 88 (69) | 24 (39) | 15 (88) | 49 (100) | <0.001 | <0.001 | ||
| papAH | 32 (25) | 27 (44) | 1 (6) | 4 (8) | 0.004 | <0.001 | ||
| papC | 38 (30) | 31 (50) | 1 (6) | 6 (12) | 0.002 | <0.001 | ||
| papEF | 36 (28) | 31 (50) | 1 (6) | 4 (8) | 0.002 | <0.001 | ||
| papG | 33 (26) | 28 (45) | 1 (6) | 4 (8) | 0.004 | <0.001 | ||
| papG II | 25 (20) | 19 (31) | 1 (6) | 5 (10) | 0.001 | |||
| Toxins | cdtB | 11 (9) | 11 (18) | 0 (0) | 0 (0) | 0.001 | ||
| hlyD | 22 (17) | 20 (32) | 1 (6) | 1 (2) | 0.03 | <0.001 | ||
| hlyF | 17 (13) | 14 (23) | 0 (0) | 3 (6) | 0.03 | 0.02 | ||
| sat | 84 (66) | 20 (32) | 15 (88) | 49 (100) | <0.001 | <0.001 | ||
| Siderophores | iron | 23 (18) | 20 (32) | 0 (0) | 3 (6) | 0.009 | <0.001 | |
| iutA | 108 (84) | 45 (72) | 15 (88) | 48 (98) | <0.001 | |||
| Protectins | cvaC | 16 (13) | 15 (24) | 0 (0) | 1 (2) | 0.032 | 0.001 | |
| iss | 25 (20) | 22 (36) | 0 (0) | 3 (6) | 0.002 | <0.001 | ||
| K2 and K100 | 54 (42) | 16 (26) | 0 (0) | 38 (78) | 0.035 | <0.001 | <0.001 | |
| K5 | 16 (13) | 2 (3) | 8 (47) | 6 (12) | <0.001 | 0.005 | ||
| kpsM II | 113 (88) | 54 (87) | 10 (59) | 49 (100) | 0.015 | 0.009 | <0.001 | |
| Invasins | ibeA | 50 (39) | 49 (79) | 0 (0) | 1 (2) | <0.001 | <0.001 | |
P values, by Fisher's (2-tailed) exact test, are shown where P < 0.05.
Traits shown in the table (where they are listed alphabetically by functional category): afa and dra (Dr family adhesins), cdtB (cytolethal distending toxin), cvaC (microcin V), hlyD (hemolysin), hlyF (hemolysin F), ibeA (invasion of brain endothelium), iha (adhesin-siderophore), iroN (siderophore receptor), iss (increased serum survival), iutA (aerobactin receptor), K2 and K100 (capsular antigen), kfiC (K5 capsular antigen), kpsM II (group 2 capsule), pap genes (operon corresponding to P fimbriae, including papAH [structural subunit], papC [assembly], papEF [tip pilins], and papG [adhesin], with allele II), sat (secreted autotransporter toxin).
Traits detected in ≥1 isolate each but not yielding a significant between-group difference (median occurrence level, 5%; range, 1% to 100%): astA (enteroaggregative heat-stable enterotoxin), cnf1 (cytotoxic necrotizing factor); fimH (type 1 fimbriae), fyuA (yersiniabactin receptor), hra (heat-resistant agglutinin), ireA (siderophore receptor), K1 capsular antigen, K15 capsular antigen, malX (pathogenicity island marker), ompT (outer membrane protease), papG allele III (cystitis-associated adhesins), sfaS (S fimbriae), traT (serum resistance associated), tsh (temperature-sensitive hemagglutinin), usp (uropathogen-specific protein).
Traits screened for but not detected in any isolate (definition, associated pathotype): afaA8, (afimbrial adhesin VIII), bmaE (M fimbriae), clbB and clbN (peptide-polyketide synthase), clpG (fimbrial adhesin CS31A), gafD (G fimbriae), f17 (F17 fimbriae), fliC (H7 flagellin), focG (F1C fimbriae), kpsMT III (group 3 capsules), papG allele I, vat (vacolating autotransporter), pic (protein involved in intestinal colonization), rfc (O4 lipopolysaccharide), sfa and focDE (S and F1C fimbriae).