FIG 1.

(A) Effect of CAS5 and RPN4 on MIC and growth on YPD agar as determined by Etest. A confluent lawn of C. albicans was streaked prior to the addition of Etest strips and then incubated for 48 h. (B) MIC heat map of SC5314, mutants, and complemented derivatives. Susceptibility was determined by broth microdilution in YPD medium at 72 h. Growth was quantified spectrophotometrically and assigned to a colorimetric scale. (C) Effect of CAS5 and RPN4 on growth on solid medium containing fluconazole. From 4-fold serial dilutions of C. albicans strains, 2-μl aliquots were spotted onto YPD agar with or without 10 μg/ml FLC and incubated for 48 h. (D) Effect of fluconazole on CAS5 and RPN4 in time-kill assays. SC5314, cas5Δ/Δ, or rpn4Δ/Δ cells were diluted in YPD medium containing FLC (10 μg/ml) or the solvent dimethyl sulfoxide (DMSO). After 0, 6, 12, and 24 h, samples from each dilution were diluted and plated for CFU. Shown are the means of data from three independent experiments with standard error bars.