TABLE 5.
Genes upregulated by ≥1.5-fold by fluconazole that are dependent upon Cas5
| Processa | orf19 designation | CGD | Fold change in expression |
|||||
|---|---|---|---|---|---|---|---|---|
| SC5314 + FLC/SC5314 (A) |
cas5Δ/Δ+ FLC/cas5Δ/Δ (B) |
B/A ratio |
||||||
| Expt 1 | Expt 2 | Expt 1 | Expt 2 | Expt 1 | Expt 2 | |||
| Lipid metabolic process | orf19.10 | ALK8 | 9.0 | 8.1 | 3.0 | 3.5 | 0.3 | 0.4 |
| orf19.2248 | ARE2 | 1.7 | 1.6 | 0.7 | 0.7 | 0.4 | 0.4 | |
| orf19.2670 | 1.6 | 1.8 | 0.4 | 0.4 | 0.3 | 0.2 | ||
| orf19.2909 | ERG26 | 2.4 | 1.7 | 0.9 | 0.9 | 0.4 | 0.5 | |
| orf19.4897 | SFH5 | 2.4 | 2.4 | 1.3 | 1.2 | 0.5 | 0.5 | |
| orf19.4982 | 2.1 | 2.3 | 0.9 | 0.6 | 0.4 | 0.3 | ||
| orf19.5640 | PEX5 | 2.2 | 2.6 | 0.9 | 0.9 | 0.4 | 0.3 | |
| orf19.5751 | ORM1 | 3.3 | 3.6 | 1.6 | 1.7 | 0.5 | 0.5 | |
| orf19.6594 | PLB3 | 2.1 | 3.4 | 1.1 | 0.9 | 0.5 | 0.3 | |
| Cell wall organization | orf19.212 | VPS28 | 1.7 | 2.1 | 0.5 | 0.5 | 0.3 | 0.3 |
| orf19.5302 | PGA31 | 17.6 | 4.1 | 2.1 | 1.5 | 0.1 | 0.4 | |
| orf19.5644 | 2.3 | 3.5 | 1.1 | 1.0 | 0.5 | 0.3 | ||
| orf19.6102 | RCA1 | 2.5 | 3.4 | 1.2 | 1.2 | 0.5 | 0.4 | |
| orf19.6420 | PGA13 | 15.6 | 30.6 | 2.4 | 2.3 | 0.2 | 0.1 | |
| orf19.6481 | YPS7 | 1.6 | 2.3 | 0.4 | 0.4 | 0.3 | 0.2 | |
| orf19.719 | 1.8 | 3.3 | 1.0 | 1.0 | 0.5 | 0.3 | ||
| Iron ion transport | orf19.1415 | FRE10 | 2.0 | 4.2 | 0.9 | 2.3 | 0.5 | 0.5 |
| orf19.1932 | CFL4 | 35.6 | 359 | 2.4 | 6.6 | 0.1 | 0.0 | |
| orf19.4215 | FET34 | 2.1 | 5.2 | 0.2 | 0.5 | 0.1 | 0.1 | |
| orf19.7219 | FTR1 | 3.2 | 7.8 | 0.5 | 1.1 | 0.1 | 0.1 | |
| Iron ion binding | orf19.1411 | 2.7 | 4.8 | 1.1 | 0.9 | 0.4 | 0.2 | |
| Iron ion homeostasis | orf19.1264 | CFL2 | 2.0 | 6.0 | 0.6 | 0.5 | 0.3 | 0.1 |
| Transport | orf19.111 | CAN2 | 1.7 | 13.0 | 0.7 | 1.5 | 0.4 | 0.1 |
| orf19.1252 | YME1 | 1.9 | 3.0 | 1.0 | 0.7 | 0.5 | 0.2 | |
| orf19.1313 | CDR3 | 1.9 | 2.1 | 0.9 | 1.0 | 0.5 | 0.5 | |
| orf19.1352 | TIM22 | 3.6 | 4.4 | 0.9 | 1.1 | 0.2 | 0.3 | |
| orf19.1563 | ECM3 | 1.5 | 1.5 | 0.4 | 0.6 | 0.3 | 0.4 | |
| orf19.1867 | 2.6 | 4.2 | 0.7 | 0.6 | 0.3 | 0.2 | ||
| orf19.2073 | 1.5 | 1.6 | 0.6 | 0.5 | 0.4 | 0.3 | ||
| orf19.2292 | OPT4 | 5.2 | 3.7 | 1.2 | 1.0 | 0.2 | 0.3 | |
| orf19.2350 | 2.5 | 4.1 | 0.6 | 1.0 | 0.3 | 0.3 | ||
| orf19.2810 | AAP1 | 18.9 | 19.6 | 7.5 | 8.8 | 0.4 | 0.4 | |
| orf19.2946 | HNM4 | 8.4 | 4.9 | 2.9 | 2.5 | 0.3 | 0.5 | |
| orf19.3015 | ARX1 | 2.7 | 3.9 | 1.3 | 1.7 | 0.5 | 0.4 | |
| orf19.3195 | HIP1 | 1.5 | 2.6 | 0.6 | 0.7 | 0.4 | 0.3 | |
| orf19.3232 | 24.6 | 5.3 | 2.6 | 1.0 | 0.1 | 0.2 | ||
| orf19.3574 | MDJ2 | 5.6 | 3.1 | 2.0 | 1.2 | 0.4 | 0.4 | |
| orf19.4041 | PEX4 | 4.9 | 4.3 | 1.3 | 1.5 | 0.3 | 0.4 | |
| orf19.4090 | 2.1 | 1.7 | 1.1 | 0.8 | 0.5 | 0.5 | ||
| orf19.4372 | 1.8 | 3.0 | 0.6 | 0.6 | 0.3 | 0.2 | ||
| orf19.4384 | HXT5 | 70.6 | 75.5 | 11.1 | 23.4 | 0.2 | 0.3 | |
| orf19.4546 | HOL4 | 4.3 | 5.1 | 1.7 | 2.3 | 0.4 | 0.4 | |
| orf19.4682 | HGT17 | 45.0 | 24.7 | 7.0 | 7.3 | 0.2 | 0.3 | |
| orf19.4887 | ECM21 | 2.7 | 3.1 | 0.8 | 1.0 | 0.3 | 0.3 | |
| orf19.5539 | 1.6 | 1.6 | 0.7 | 0.8 | 0.5 | 0.5 | ||
| orf19.5902 | RAS2 | 7.6 | 6.2 | 0.8 | 1.1 | 0.1 | 0.2 | |
| orf19.5958 | CDR2 | 2.1 | 2.5 | 0.9 | 1.0 | 0.4 | 0.4 | |
| orf19.6117 | 1.5 | 2.3 | 0.6 | 0.4 | 0.4 | 0.2 | ||
| orf19.6249 | HAK1 | 4.4 | 10.7 | 2.1 | 3.2 | 0.5 | 0.3 | |
| orf19.6648 | SDA1 | 3.2 | 6.8 | 1.6 | 3.2 | 0.5 | 0.4 | |
| orf19.6993 | GAP2 | 21.7 | 8.6 | 3.0 | 4.5 | 0.1 | 0.5 | |
| orf19.7056 | 6.2 | 120.3 | 2.5 | 2.7 | 0.4 | 0.0 | ||
| orf19.7093 | HGT13 | 30.7 | 13.2 | 2.0 | 1.5 | 0.1 | 0.1 | |
| orf19.7094 | HGT12 | 423.2 | 19.8 | 8.0 | 1.9 | 0.0 | 0.1 | |
| Response to chemical stimulus | orf19.2060 | SOD5 | 33.2 | 14.2 | 5.4 | 6.1 | 0.2 | 0.4 |
| orf19.2838 | 2.5 | 2.9 | 0.8 | 0.7 | 0.3 | 0.3 | ||
| orf19.2876 | CBF1 | 2.3 | 3.2 | 0.5 | 0.5 | 0.2 | 0.2 | |
| orf19.3159 | UTP20 | 3.8 | 8.9 | 1.9 | 3.5 | 0.5 | 0.4 | |
| orf19.3736 | KAR4 | 2.8 | 2.6 | 1.2 | 1.0 | 0.4 | 0.4 | |
| orf19.4015 | CAG1 | 3.1 | 3.6 | 0.8 | 0.7 | 0.3 | 0.2 | |
| orf19.4317 | GRE3 | 1.5 | 1.5 | 0.8 | 0.6 | 0.5 | 0.4 | |
| orf19.4318 | MIG1 | 1.7 | 2.1 | 0.7 | 1.0 | 0.4 | 0.5 | |
| orf19.5326 | 1.5 | 1.9 | 0.6 | 0.7 | 0.4 | 0.4 | ||
| orf19.5591 | ADO1 | 2.4 | 2.9 | 01.1 | 1.5 | 0.4 | 0.5 | |
| orf19.6202 | RBT4 | 3.3 | 6.2 | 1.6 | 2.2 | 0.5 | 0.3 | |
| orf19.6881 | YTH1 | 1.9 | 2.0 | 0.9 | 0.9 | 0.4 | 0.4 | |
| orf19.7316 | 2.0 | 2.3 | 0.7 | 0.8 | 0.3 | 0.3 | ||
| orf19.7384 | NOG1 | 3.7 | 7.2 | 1.2 | 2.1 | 0.3 | 0.3 | |
| Filamentous growth | orf19.2397.3 | 2.0 | 4.3 | 0.9 | 1.2 | 0.5 | 0.3 | |
| orf19.4055 | 1.9 | 2.5 | 0.9 | 0.7 | 0.5 | 0.3 | ||
| orf19.4815 | YTM1 | 2.3 | 3.2 | 0.6 | 1.4 | 0.3 | 0.4 | |
| orf19.4928 | SEC2 | 2.0 | 2.1 | 0.4 | 0.4 | 0.2 | 0.2 | |
| orf19.5741 | ALS1 | 66.8 | 79.0 | 22.6 | 21.0 | 0.3 | 0.3 | |
| orf19.5798 | LIG4 | 1.8 | 2.6 | 0.9 | 0.8 | 0.5 | 0.3 | |
| orf19.6595 | RTA4 | 10.4 | 15.0 | 3.0 | 2.9 | 0.3 | 0.2 | |
| orf19.6888 | 4.3 | 5.7 | 1.4 | 1.1 | 0.3 | 0.2 | ||
| orf19.7313 | SSU1 | 4.5 | 4.8 | 1.3 | 1.1 | 0.3 | 0.2 | |
| orf19.7374 | CTA4 | 1.7 | 2.2 | 0.8 | 1.1 | 0.5 | 0.5 | |
| orf19.7436 | AAF1 | 2.0 | 4.4 | 1.0 | 1.2 | 0.5 | 0.3 | |
| orf19.795 | VPS36 | 2.0 | 2.5 | 0.6 | 0.6 | 0.3 | 0.3 | |
| Oxidation-reduction process | orf19.1117 | 5.7 | 2.3 | 0.7 | 1.1 | 0.1 | 0.5 | |
| orf19.1473 | 35.0 | 15.0 | 3.8 | 4.8 | 0.1 | 0.3 | ||
| orf19.2108 | SOD6 | 7.9 | 10.6 | 1.6 | 1.1 | 0.2 | 0.1 | |
| orf19.3538 | FRE9 | 4.7 | 14.9 | 2.5 | 3.6 | 0.5 | 0.2 | |
| orf19.3707 | YHB1 | 3.0 | 2.8 | 0.9 | 0.6 | 0.3 | 0.2 | |
| orf19.4747 | HEM14 | 1.7 | 2.0 | 0.6 | 0.4 | 0.3 | 0.2 | |
| orf19.4871 | ERO1 | 1.9 | 1.9 | 0.9 | 0.6 | 0.5 | 0.3 | |
| orf19.5879 | 3.3 | 2.9 | 1.5 | 1.2 | 0.5 | 0.4 | ||
| orf19.638 | FDH1 | 22.7 | 19.5 | 4.4 | 3.9 | 0.4 | 0.2 | |
| orf19.6837 | FMA1 | 1.5 | 1.5 | 0.7 | 0.7 | 0.5 | 0.4 | |
| orf19.7111.1 | SOD3 | 40.5 | 69.2 | 9.4 | 15.0 | 0.2 | 0.2 | |
| orf19.7314 | CDG1 | 7.5 | 6.0 | 1.0 | 0.6 | 0.1 | 0.1 | |
| orf19.742 | ALD6 | 11.5 | 10.4 | 4.1 | 1.7 | 0.4 | 0.2 | |
| orf19.7551 | ALO1 | 1.5 | 2.8 | 0.7 | 0.6 | 0.5 | 0.2 | |
a
Descriptions are from the Candida Genome Database (http://www.candidagenome.org/).