FIG 1.

Permissivity of COS-7, HEK 293TT, SVG-A, and M03.13 cells for JCPyV MAD-4. The cells were infected with JCPyV MAD-4 for 2 h before the inoculum was replaced with fresh medium. (A) Extra- and intracellular JCPyV DNAs were quantified by qPCR of supernatants and extracted DNA from cell lysates, respectively, harvested at the indicated time points. The mean values (GEq/ml) ± standard deviations (SDs) of two parallel samples from one representative experiment are presented. (B) At 4 days postinfection, the cells were fixed and immunofluorescence staining performed using as primary antibody polyclonal rabbit anti-BKPyV LTag C-terminal serum that cross-reacts with SV40 LTag and JCPyV LTag (green) and a monoclonal mouse antibody directed against JCPyV VP1 (red). The cell nuclei (blue) were stained with Draq5. The pictures were taken with a fluorescence microscope (×10 objective).