FIG 5.

Cytotoxicity of artesunate-treated mock-infected and JCPyV-infected COS-7 cells. Mock-infected (A) and JCPyV-infected (B) COS-7 cells were treated with the indicated concentrations of artesunate containing the CellTox green dye from 2 hpi. This dye has increased fluorescence intensity when bound to the DNA of cells with an impaired cell membrane. The fluorescence intensity of the dye was measured at 24, 48, 72, and 96 hpt. To correlate the cell death found in artesunate-treated cells with that in the untreated cells, the fluorescence intensity of each drug concentration measured at all time points was divided by that of untreated mock-infected cells at 24 hpt. The mean values ± SDs from two individual experiments are presented as fold changes, and each experiment was performed in 3 parallel wells.