Growth (A), normalized gfp expression (B), and normalized pyoverdine production (C) of the P. aeruginosa PAO1 biosensor strain carrying pvdA-gfp in ABTGC medium containing various concentrations of iberin. Every 30 min, growth, gfp fluorescence, and pyoverdine production were measured using OD600 values and relative fluorescence units (RFU), respectively. Normalization was calculated as RFU divided by OD600. The experiments were performed in triplicate; only representative ones are shown.