TABLE 2.
Primers used in this study
| Primer | Sequence | Target gene and purpose | Reference |
|---|---|---|---|
| 16S_RT_F | ACATCTCACGACACGAGCTG | 16S rRNA, gene expression | 38 |
| 16S_RT_R | CGTAAGGGCCATGATGACTT | ||
| adeB_RT_F | GGATTATGGCGACTGAAGGA | adeB, gene expression | 38 |
| adeB_RT_R | AATACTGCCGCCAATACCAG | ||
| adeG_RT_F | CGTAACTATGCGGTGCTCAA | adeG, gene expression | 38 |
| adeG_RT_R | ATCGCGTAGTCACCAGAACC | ||
| adeJ_RT_F | CATCGGCTGAAACAGTTGAA | adeJ, gene expression | 38 |
| adeJ_RT_R | GCCTGACCATTACCAGCACT | ||
| A1S_3217_RT_F | ACCGCTTTAGAGGTCGAACA | A1S_3217, gene expression | 38 |
| A1S_3217_RT_R | GTGACTTGGGAAAGCCCATA | ||
| A1S_2818_RT_F | AATTGAGCCAAGCTCATGCT | A1S_2818, gene expression | 38 |
| A1S_2818_RT_R | TCCGCGATGAAATTGATACA | ||
| carO_RT_F | AGCAGTTCGTGGTCAAGAGG | carO, gene expression | 38 |
| carO_RT_R | TTGGAGCAAAACCAAAACCT | ||
| oprD_RT_F | CCAGCTCAGTTGCTCAATCA | oprD, gene expression | 38 |
| oprD_RT_R | AACAACGCCTACACCGAAAC | ||
| abeM_RT_F | TGCCAATTGGTTTAGCTGTG | abeM, gene expression | This study |
| abeM_RT_R | TACTTGGTGTGCGGCAATAA | ||
| adeN_RT_F | CAACCTGAACACATTGCCTTT | adeN, gene expression | This study |
| adeN_RT_R | TTTTGGACATCCAGAGCACA | ||
| fabI_RT_F | TTTAGAAGCTGGCGTTCGTT | fabI, gene expression | This study |
| fabI_RT_R | AGCAGCCAAAGTACGGATTG | ||
| fabI_FL_F | GTGAGATCGGCATGACACAA | fabI, cloning and sequencing of fabIa | This study |
| fabI_FL_R | ATAACGGTAGCGGAGTTCAG | ||
| adeN_FL_R | AGTCTACTATACTATAAGCATTTC | adeN, cloning and sequencing of adeNb | This study |
| adeN_ FL_F | GATAAGCAGTGTTAGCCGTCG | ||
| adeIJK_PR_F | CTTCAGAAATTTGATATGCT | adeIJK promoter, sequencingc | This study |
| adeIJK_PR_R | GATTATGTTATGCCATAAGC | ||
| adeI_For_Sp | TTACTAGTTATCTAAACGAGGTGd | adeI, adeJ, cloning of adeIJ′ fragment (4,051 bp) | This study |
| adeJ_Rev_Kp | TCAATACGATTGCACCAATGAC | ||
| adeJ_For_Kp | TATATGAAAGCTGGTCAATTCCG | adeJ, adeK, cloning of adeJ′K fragment (1,983 bp) | This study |
| adeK_Rev_Xh | CCCACCGACTCGAGCTTTTATAAGe | ||
| PaglmS_Dn | GCACATCGGCGACGTGCTCTC | glmS and pUC18T-miniTn7T-Gm-LAC, confirmation of the insertion of mini-Tn7 element in P. aeruginosa | 24 |
| Tn7R | CACAGCATAACTGGACTGATTTC |
a
Primers bind 233 bp upstream of the start and 11 bp downstream of stop codons, respectively.
b
Primers bind 32 bp upstream of the start and 38 bp downstream of stop codons, respectively.
c
Promoter/sequencing of the promoter region 244 bp upstream and 220 bp downstream of the start codon of the adeI gene.
d
Engineered SpeI site is underlined, and introduced base changes are shown in bold type.
e
Engineered XhoI site is underlined, and introduced base changes shown are bold.