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. 2014 Dec;58(12):7527–7533. doi: 10.1128/AAC.03169-14

FIG 1.

FIG 1

(A) Killing of M. tuberculosis H37Rv by RIF generated an increase in the rate of CPH radical formation. Shown are M. tuberculosis survival (log10 CFU/ml) and rate of CPH radical formation (μM/min) after 1 day (D1) and 3 days (D3) of treatment with RIF. Means and standard deviations (SD) from three experiments are shown. (B) Rate of CPH radical formation (μM/min ± SD) after 3 days of incubation of M. tuberculosis H37Rv alone (H37Rv) and in the presence of 0.125, 0.5, 2, 8 μg/ml RIF, with or without pretreatment of the lysates with 10 μg/ml SOD, 100 mM THIO, 1 mM DTPA. (C) Rate of CPH radical formation (μM/min ± SD) after 3 days of incubation of M. tuberculosis H37Rv with 8 μg/ml RIF or 4 μg/ml EMB and of RIF-resistant H37Rv (H37Rv RIF-R) with 8 μg/ml RIF or 4 μg/ml MX. (D) Rate of CPH radical formation (μM/min ± SD) after 1 day of incubation of RIF-susceptible (strains 5502 and 5050) and RIF-resistant (strains 7225, 3063, and 4164) clinical isolates, alone and in the presence of 8 μg/ml RIF. RIF-S, RIF susceptible; RIF-R, RIF resistant.