Map of pSN254b. Genes are represented by arrows. Genes on the outside are transcribed clockwise, whereas genes on the inside are transcribed counterclockwise. Red, green, and blue arrows represent genes coding for antibiotic resistance, hypothetical proteins, and other functions, respectively. Gray rectangles represent mobile elements, such as transposons and IS. The inner ring represents the GC skew calculated by DNAPlotter with a window and step size of 20,000 bp and 300 bp, respectively. Each tick mark represents a 5,000-bp step.