TABLE 1.
Isolatea | Antibiotic resistanceb | MIC (μg/ml) for tetracyclinec | Plasmids foundd |
---|---|---|---|
01-B522 | SXT, TET, TMP | 128 | Cryptic, pAsal1, pAsa5, pAsa4-like |
07-9324 | STR, AMP, TET, CHL | 16 | Cryptic, pAsal1, pAsa5, pSN254-like |
07-7817 | STR, AMP, TET, CHL | 16 | Cryptic, pAsal1, pAsa5, pSN254-like |
08-2647 | SXT, STR, AMP, TET | 16 | Cryptic, pAsal1, pAsa5, pSN254-like |
07-7287 | STR, AMP, TET, CHL | 16 | Cryptic, pAsal1, pAsa5, pSN254-like |
08-2783 | STR, AMP, TET | 16 | Cryptic, pAsal1, pAsa5, pSN254-like |
08-4188 | STR, AMP, TET, CHL | 16 | Cryptic, pAsal1, pAsa5, pSN254-like |
2009-157K5 | STR, TET, ERY | 128 | Cryptic, pAsal1, pAsa5, pRas3.3 |
2010-47K18 | STR, TET, CHL | 128 | Cryptic, pAsal1, pAsa5, pRas3.4, pAB5S9-like |
2004-05MF26 | NAL, SXT, STR, AMP, TET, ERY, GEN, CHL | 16 | Cryptic, pAsal1, pAsa5, pSN254b |
2004-68K52 | NAL, SXT, STR, AMP, TET, CHL | 64 | Cryptic, pAsal1, pAsa5, pSN254-like |
2009-195K29 | STR, TET, ERY | 32 | Cryptic, pAsal1, pAsa5, pRas3.3 |
2009-144K3 | SXT, STR, TET, ERY, CHL | 256 | Cryptic, pAsal1, pAsa5, pRas3.3, pAB5S9b |
M15448-11 | STR, AMP, TET, CHL | 32 | Cryptic, pAsal1, pAsa5 |
M16474-11 | STR, AMP, TET, ERY, CHL | 32 | Cryptic, pAsal1, pAsa5 |
M14481-11 | SXT, STR, AMP, TET, CHL | 16 | Cryptic, pAsal1, pAsa5, pSN254-like |
M15879-11 | SXT, STR, AMP, TET, CHL | 16 | Cryptic, pAsal1, pAsa5, pSN254-like |
M17739-11 | SXT, STR, AMP, TET, CHL | 16 | Cryptic, pAsal1, pAsa5, pSN254-like |
M13732-11 | STR, AMP, TET, CHL | 16 | Cryptic, pAsal1, pAsa5, pSN254-like |
M17053-11 | STR, AMP, TET, CHL | 16 | Cryptic, pAsal1, pAsa5, pSN254-like |
M15469-11 | STR, AMP, TET, CHL | 16 | Cryptic, pAsal1, pAsa5, pSN254-like |
The origin and source of these isolates are given in Table S1 in the supplemental material.
NAL, nalidixic acid; SXT, sulfamethoxazole-trimethoprim; STR, streptomycin; AMP, ampicillin; TET, tetracycline; ERY, erythromycin; GEN, gentamicin; TMP, trimethoprim; CHL, chloramphenicol.
The MIC of the sensitive 01-B526 isolate was 0.5 μg/ml.
The three cryptic plasmids pAsa1, pAsa2, and pAsa3, as well as pAsal1 and pRAS3, were identified in these isolates in a previous study by Trudel et al. (9). The pAsa5, pAsa4-like, pAB5S9-like, and pSN254-like plasmids were found by PCR genotyping in the present study (see Table S2 in the supplemental material).