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In vitro translation of MCV and VV polymerases and processivity factors. The E9 polymerases and the processivity factors D4 and A20 from the molluscum contagiosum and vaccinia viruses were translated in vitro from cloned plasmids and labeled with [35S]Cys/Met. Proteins were fractionated on an SDS gel and visualized by autoradiography. Arrows indicate full-length proteins. Note that lanes from the original autoradiogram were rearranged for convenient comparison.
