TABLE 1.
Interactions of voriconazole, amphotericin B, and caspofungin against biofilm and planktonic forms of 16 clinical isolates of T. asahiia
| Isolate no. | SMIC (μg/ml) |
FICI | SMIC (μg/ml) for VRZ/CAS | FICI | SMIC (μg/ml) for AMB/CAS | FICI | MIC (μg/ml) |
FICI | MIC (μg/ml) for VRZ/CAS | FICI | MIC (μg/ml) for AMB/CAS | FICI | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| VRZ | AMB | CAS | VRZ/AMB | VRZ | AMB | CAS | VRZ/AMB | |||||||||||
| 1 | 512 | 128 | 64 | 4/16 | 0.133 | 2/16 | 0.254 | 2/32 | 0.516 | 0.0625 | 2 | 16 | 0.0313/0.5 | 0.75 | 0.0313/0.25 | 0.516 | 0.5/4 | 0.5 |
| 2 | 512 | 256 | 64 | 4/64 | 0.258 | 4/32 | 0.508 | 0.5/64 | 1.002 | 0.0625 | 8 | 32 | 0.0625/4 | 1.5 | 0.0313/2 | 0.625 | 2/1 | 0.281 |
| 3 | >1,024 | 512 | 32 | 16/32 | 0.078 | 8/16 | 0.508 | 0.5/32 | 1.001 | 0.125 | 8 | 16 | 0.0313/1 | 0.375 | 0.0313/0.5 | 0.281 | 0.5/2 | 0.188 |
| 4 | >1,024 | 512 | 64 | 8/32 | 0.07 | 2/32 | 0.502 | 0.5/64 | 1.001 | 0.125 | 4 | 32 | 0.0625/4 | 1.5 | 0.0625/0.5 | 0.516 | 0.125/4 | 0.156 |
| 5 | >1,024 | 512 | 64 | 64/4 | 0.07 | 2/32 | 0.502 | 4/32 | 0.508 | 0.0625 | 4 | 32 | 0.0157/4 | 1.35 | 0.0625/1 | 1.031 | 0.5/1 | 0.156 |
| 6 | >1,024 | 32 | 32 | 32/16 | 0.531 | 1/32 | 1.001 | 0.5/32 | 1.016 | 0.0625 | 4 | 16 | 0.0313/1 | 0.75 | 0.0313/1 | 0.563 | 0.25/8 | 0.625 |
| 7 | >1,024 | 256 | 128 | 8/128 | 0.508 | 4/32 | 0.254 | 1/64 | 1.004 | 0.125 | 2 | 16 | 0.125/2 | 2 | 0.0313/0.25 | 0.375 | 0.5/2 | 0.375 |
| 8 | >1,024 | 128 | 64 | 4/16 | 0.129 | 2/32 | 0.502 | 4/32 | 0.531 | 0.0625 | 1 | 8 | 0.0625/0.5 | 1.5 | 0.0625/0.5 | 1.063 | 0.0625/2 | 0.313 |
| 9 | 1,024 | 256 | 64 | 4/32 | 0.129 | 4/32 | 0.504 | 1/64 | 1.004 | 0.0313 | 2 | 32 | 0.0313/0.25 | 1.25 | 0.0157/4 | 0.625 | 0.25/1 | 0.156 |
| 10 | >1,024 | 1,024 | 64 | 128/8 | 0.133 | 2/32 | 0.502 | 1/32 | 0.501 | 0.0313 | 2 | 32 | 0.0313/1 | 1 | 0.0625/2 | 1.125 | 1/8 | 0.75 |
| 11 | >1,024 | 1,024 | 32 | 64/16 | 0.078 | 0.5/32 | 1 | 0.5/32 | 0.5 | 0.0625 | 4 | 16 | 0.0313/4 | 2 | 0.0313/0.25 | 1.016 | 0.5/2 | 0.25 |
| 12 | >1,024 | 1,024 | 64 | 32/128 | 0.156 | 1/32 | 0.501 | 1/64 | 1.001 | 0.0625 | 4 | 16 | 0.0313/1 | 1.25 | 0.0313/0.5 | 1.031 | 0.5/4 | 0.375 |
| 13 | >1,024 | 128 | 64 | 16/16 | 0.281 | 2/16 | 0.252 | 2/16 | 0.266 | 0.0313 | 8 | 16 | 0.0313/2 | 0.75 | 0.0625/0.25 | 1.016 | 1/1 | 0.188 |
| 14 | >1,024 | 1,024 | 64 | 128/32 | 0.156 | 16/32 | 0.516 | 2/64 | 1.002 | 0.0625 | 8 | 32 | 0.0625/8 | 2 | 0.0313/1 | 0.531 | 0.5/2 | 0.125 |
| 15 | 1,024 | 64 | 64 | 16/8 | 0.141 | 0.5/16 | 0.25 | 0.5/64 | 1.008 | 0.0313 | 8 | 32 | 0.0313/2 | 1.25 | 0.0313/1 | 1.031 | 0.25/8 | 0.281 |
| 16 | >1,024 | 128 | 32 | 64/32 | 0.313 | 2/32 | 1.001 | 1/32 | 1.008 | 0.0625 | 4 | 16 | 0.0313/1 | 0.75 | 0.0157/0.5 | 0.281 | 2/2 | 0.625 |
a
VRZ, voriconazole; AMB, amphotericin B; CAS, caspofungin. SMIC, sessile MIC, defined as the concentration that causes a 50% reduction in optical density of the biofilms compared with the optical density of the untreated biofilm formed by the same isolates; MIC, the concentration causing a 50% reduction in optical density of the planktonic cells compared with the optical density of the untreated cells of the same isolates; FICI, fractional inhibitory concentration index: ≤0.5, synergy; >0.5 to 4, indifference; >4.0, antagonism.