TABLE 6.
Chromosomal cephalosporinase (AmpC), efflux pumps, and outer membrane protein expression levels among carbapenemase-negative Enterobacteriaceae isolates collected from European and Mediterranean countries during 2009 to 2011a
| Yr | Site code | Country | Organism | PFGE result | Relative expressionb |
||||
|---|---|---|---|---|---|---|---|---|---|
| acrA | ampC | ompK35/ompF | ompK36/ompC | ompK37 | |||||
| 2009 | 081 | Poland | E. cloacae | ECL81C | 4.167 | 82.660 | 4.233 | 0.006 | NT |
| 2010 | 063 | Israel | K. pneumoniae | KPN63F | 4.369 | 2.203 | 0.970 | 1.292 | 0.635 |
| 2010 | 063 | Israel | K. pneumoniae | KPN63F | 1.886 | 0.953 | 0.718 | 0.011 | 0.612 |
| 2010 | 081 | Poland | K. pneumoniae | KPN81F | 1.562 | 1.216 | 0.468 | 1.033 | 0.736 |
| 2011 | 063 | Israel | K. pneumoniae | KPN63E | 0.999 | 1.108 | 6.478 | 0.020 | 0.826 |
| 2011 | 081 | Poland | K. pneumoniae | KPN81B | 6.587 | 1.800 | 0.193 | 0.000 | 0.472 |
a
One C. freundii isolate from Belgium was not analyzed because it failed in assays designed with available DNA sequences for the intrinsic genes tested.
b
Expression was normalized against that of a housekeeping gene (rspL for E. cloacae and gyrA for K. pneumoniae) and compared to expression of E. cloacae ATCC 700323 and K. pneumoniae ATCC 13883. Expression values considered significant are underlined. NT, not tested.