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. 2014 Dec;58(12):7358–7366. doi: 10.1128/AAC.03930-14

TABLE 6.

Chromosomal cephalosporinase (AmpC), efflux pumps, and outer membrane protein expression levels among carbapenemase-negative Enterobacteriaceae isolates collected from European and Mediterranean countries during 2009 to 2011a

Yr Site code Country Organism PFGE result Relative expressionb
acrA ampC ompK35/ompF ompK36/ompC ompK37
2009 081 Poland E. cloacae ECL81C 4.167 82.660 4.233 0.006 NT
2010 063 Israel K. pneumoniae KPN63F 4.369 2.203 0.970 1.292 0.635
2010 063 Israel K. pneumoniae KPN63F 1.886 0.953 0.718 0.011 0.612
2010 081 Poland K. pneumoniae KPN81F 1.562 1.216 0.468 1.033 0.736
2011 063 Israel K. pneumoniae KPN63E 0.999 1.108 6.478 0.020 0.826
2011 081 Poland K. pneumoniae KPN81B 6.587 1.800 0.193 0.000 0.472
a

One C. freundii isolate from Belgium was not analyzed because it failed in assays designed with available DNA sequences for the intrinsic genes tested.

b

Expression was normalized against that of a housekeeping gene (rspL for E. cloacae and gyrA for K. pneumoniae) and compared to expression of E. cloacae ATCC 700323 and K. pneumoniae ATCC 13883. Expression values considered significant are underlined. NT, not tested.