FIG 2.

Omeprazole inactivates GlTIM by means of interaction with Cys at position 222. (A) Each single mutant of Cys is inactivated in the same way as wt GlTIM, except for that without Cys 222 (for 2 h at 37°C). (B) C222A resists inactivation with omeprazole either under acid-activated and nonactivated conditions. The specific activities at baseline were 3,870, 4,006, 3,994, 3,204, 3,853, and 4,201 μmol min⁻¹ mg⁻¹ for wt-GlTIM, C14A, C127A, C202A, C222A, and C228A, respectively. The values are means ± SD from at least three independent experiments.