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. 2014 Dec;58(12):7072–7082. doi: 10.1128/AAC.02900-14

FIG 3.

FIG 3

Fluorescence of GlTIM-omeprazole adducts. (A) Top, gel without staining and photographed in a UV transilluminator. Bottom, same gel stained with Coomassie brilliant blue. Lane 1, GlTIM without omeprazole; lanes 2 to 6, GlTIM plus 0.1, 0.2, 0.5, 0.75, and 2.5 mM omeprazole, respectively. (B) Gel without staining and photographed in a UV transilluminator. Lane 1, C222A without omeprazole; lanes 2 to 6, C222A plus 0.7 mM omeprazole at 15, 30, 60, 90, and 120 min, respectively; and lane 7, HsTIM without omeprazole; lane 8, HsTIM plus 0.7 mM omeprazole at 120 min. Each lane was loaded with 10 μg of enzyme. (C) Emission spectra of recombinant GlTIM (0.6 mg/ml protein), plotted for data before (filled squares) and after (circles) incubation with 5.6 mM omeprazole (OMP). a.u., absorbance units.