FIG 4.

Biofilms treated with sub-MIC vancomycin contain more eDNA than control biofilms. (A) Average rate of activity (OD₆₀₀ min⁻¹) over 180 min for a 100-μg/ml DNase treatment to control and sub-MIC-vancomycin-treated S. epidermidis biofilms. The rate of DNase I activity was calculated as a change in OD₆₀₀ over a change in time. (B) Concentration of eDNA extracted from control and sub-MIC-vancomycin-treated biofilms. Error bars, standard errors of the mean, n = 3. Asterisks indicate statistically significant difference (P < 0.05) as determined by the Student t test.