TABLE 5.
Inhibition of incorporation of radioactive precursors specific for certain pathways in E. coli W3110 with inactivated tolCa
| Biosynthetic pathway | Radioactive precursor | IC50b (μg/ml) |
||
|---|---|---|---|---|
| Compound B | Compound C | Compound D | ||
| DNA | [3H]thymidine | 0.8 | 3 | 2 |
| RNA | [3H]uridine | >256 | 160 | >256 |
| Protein | [14C]leucine | >256 | >256 | >256 |
| Cell wall | [14C]N-acetylglucosamine | >256 | 130 | >256 |
| Fatty Acid | [14C]acetate | >256 | 120 | >256 |
a
The radioactive precursors used have been described previously (37).
b
IC50, the concentration at which incorporation of the precursor is inhibited by 50%.