GCV prevents the progression of adenoviral infection into the late phase. (A) Immunofluorescent staining for an early (DBP) and a late (hexon) Ad protein in GCV-treated or untreated A549 cells infected with species C (Ad5, Ad6), B (Ad7, Ad35), or E (Ad4) Ads. Uninfected cells did not stain with either antibody (data not shown). All images were taken with the same exposure settings. (B) Immunoblot staining for DBP and pVIII (another late Ad protein) in cells infected with the same types of Ads used for the assay whose results are shown in panel A. M.W., molecular weight markers (numbers on the right are in thousands).