TABLE 3.

Tissue concentrations of ATV and RTV, after multiple doses of FA-nanoATV/r and native-drug administration at 20 mg/kg in mice (n = 5)

Tissue	Sample collectiond	Mean concn (ng/g or μg/ga) in tissue ± SEM
		FA-nanoATV	Native ATV	FA-nanoRTV	Native RTV
Liver	Day 35, special dietb	1,314.2 ± 121.7	219.6 ± 35.1	108.9 ± 19.2	0.5 ± 0.3
	Day 70, biweekly special dietc	260.1 ± 40.6	55.1 ± 11.6	6.9 ± 2.3	0.5 ± 0.3
	Day 35, normal diet	87.7 ± 7.3	72.3 ± 10.7	44.1 ± 4.2	13.4 ± 5.8
	Day 70, biweekly normal diet	49.0 ± 14.5	18.2 ± 3.1	9.9 ± 3.6	6.5 ± 6.5
Spleen	Day 35, special diet	62.9 ± 11.2	15.2 ± 3.0	75 ± 17.6	4.2 ± 1.0
	Day 70, biweekly special diet	9.1 ± 0.4	4.5 ± 0.5	4.3 ± 1.1	2.1 ± 0.3
	Day 35, normal diet	11.6 ± 0.7	10.6 ± 0.9	33.2 ± 3.1	14.4 ± 6.3
	Day 70, biweekly normal diet	5.8 ± 1.0	2.2 ± 0.2	5.9 ± 1.4	5.3 ± 4.1
Kidney	Day 35, special diet	126.5 ± 14.4	31.5 ± 2.8	103.2 ± 11.5	3.7 ± 0.6
	Day 70, biweekly special diet	33.0 ± 4.1	11 ± 1.3	9.7 ± 3.0	3.2 ± 0.9
	Day 35, normal diet	17.5 ± 1.7	23.2 ± 7.3	69.4 ± 7.1	21.3 ± 7.5
	Day 70, biweekly normal diet	7.6 ± 0.9	5.9 ± 0.5	10.8 ± 3.2	8.1 ± 6.5
Lung	Day 35, special diet	103.2 ± 35.7	28 ± 8.8	31.3 ± 10.5	2.2 ± 1.0
	Day 70, biweekly special diet	21.7 ± 2.7	6.9 ± 1.2	2.3 ± 0.7	0.9 ± 0.2
	Day 35, normal diet	15.7 ± 2.3	13.8 ± 2.2	15.9 ± 2.3	4.9 ± 2.0
	Day 70, biweekly normal diet	65.8 ± 47.5	3.1 ± 0.4	2.4 ± 0.7	1.8 ± 1.3
Lymph node	Day 35, special diet	36.1 ± 3.5	21.8 ± 2.7	23.6 ± 7.2	4.3 ± 1.5
	Day 70, biweekly special diet	26.0 ± 1.7	20.8 ± 11.6	1.9 ± 0.7	1.2 ± 0.3
	Day 35, normal diet	17.8 ± 3.6	16.6 ± 11.1	25.4 ± 8.7	9.1 ± 6.4
	Day 70, biweekly normal diet	21.8 ± 12.9	19.5 ± 8.7	10 ± 6.3	3.1 ± 1.1
Brain	Day 35, special diet	2.78 ± 0.75	2.15 ± 0.5	0.8 ± 0.27	—e
	Day 70, biweekly special diet	1.14 ± 0.26	—	—	—
	Day 35, normal diet	0.29 ± 0.07	0.68 ±	0.4 ± 0.08	—
	Day 70, biweekly normal diet	—	—	—	—
Site of injection	Day 35, special diet	1,145.0 ± 127.8	454.4 ± 32.7	51.0 ± 17.7	9.0 ± 0.5
	Day 70, biweekly special diet	345.8 ± 69.4	563.3 ± 191.4	0.1 ± 0.0	11.6 ± 3.4
	Day 35, normal diet	847.0 ± 278.0	286 ± 50.5	11.8 ± 5.9	5.4 ± 0.7
	Day 70, biweekly normal diet	918.5 ± 161	302.6 ± 123.5	6.9 ± 5.9	4.8 ± 2.1

^aValues for site of injection are in micrograms per gram.

^bTissue samples were collected on day 35, i.e., 7 days after the last dose in the weekly-dosing group.

^cTissue samples were collected on day 70, i.e., 14 days after the last dose in the biweekly-dosing group.

^dSpecial diet is the folate-deficient diet.

^e—, values are less than the limit of quantitation (i.e., 0.5 ng/ml).