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. 2014 Aug 8;13:107. doi: 10.1186/s12934-014-0107-2

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© Koivuranta et al.; licensee BioMed Central 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Xylose consumption and L-lactic acid production in anaerobic cultivation. Concentration (g l⁻¹) of D-xylose consumed (A) and L-lactic acid produced (B) in anaerobic cultivations of C. sonorensis ldhL strains with the native D-xylose pathway disrupted and expressing XYLA alone (C311, XYLA, xyl1Δ; C313, 2*XYLA, xyl1Δ) or together with ScXKS1 (C341-C342, XYLA, ScXKS1, xyl1Δ; C345-C346, XYLA, ScXKS1, xyl1Δ, xyl2Δ; C348, 2*XYLA, ScXKS1, xyl1Δ; C349, 2*XYLA, ScXKS1, xyl1Δ, xyl2Δ), with C29 as the control. Strains were cultivated at 30°C in 50 ml of CaCO₃-buffered, YP-50 g l⁻¹ D-xylose with 10 mM MgCl₂ in 100 ml flasks sealed with water locks for 146 h. Initial OD600 was 12. Results are averages of duplicate flasks. Error bars indicate sem. Bars with the same letter (a to d) in the same graph did not differ significantly (p > 0.05).