Table 4.
Xylose reductase and xylitol dehydrogenase activities in xyl1Δ and xyl2Δ strains
| Strains | Genotype | Xylose reductase activity | Xylitol dehydrogenase activity |
|---|---|---|---|
| mU [mg protein] −1 | mU [mg protein] −1 | ||
| wild type | 75 ± 1.6 | 571 ± 21 | |
| C689, C690 | xyl1Δ | 2 ± 0.3 | 625 ± 47 |
| C684, C685, C686 | xyl2Δ | 74 ± 0.9 | 126 ± 4 |
NADPH-dependent xylose reductase and xylitol dehydrogenase activities in C. sonorensis strains in which the XYL1 or XYL2 gene were disrupted. Wild type C. sonorensis, xyl1Δ strains (C689 and C690) and xyl2Δ strains (C684, C685 and C686) were grown in YNB with 50 g l−1 D-xylose for 24 h. Results are mean ± sem for duplicate activity measurements of wild type strain, two xyl1Δ strains or three xyl2Δ strains.