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. 2014 Aug 12;13:115. doi: 10.1186/s12934-014-0115-2

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© Ricca et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Strategy of non-recombinant spore-surface display. Purified spores and antigen are reacted at 25°C in an acidic reaction buffer (pH 4.0). After the adsorption reaction spore-bound and free antigen are separated by centrifugation.