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. 2014 Aug 8;9:126. doi: 10.1186/s13023-014-0126-4

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© Otte et al. ; Licensee Biomedcentral Ltd 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Comparison of protein expression in SCCOHT-1 and NIH:OVACAR-3 cells. Western blot analysis was performed in SCCOHT-1^GFP and NIH:OVCAR-3^GFP, to compare the protein expression levels of p53, tubulinβ3, and phosphorylated heat shock protein (HSP27) at serine-82 after a 24 h incubation of the 2 cell types with 2 nM of ixabepilone (Ixa), epothilone B (EpoB), methotrexate (MTX), and 5’-fluorouracil (5FU), respectively. The unaltered expression of GAPDH served as a control for equal loading, respectively. Quantification of the blots by densitometry scanning was normalized against the appropriate GAPDH expression and the relative expression levels were documented as bar diagram below the corresponding Western blots.