Table II.
Primers used in RT-qPCR.
| Genes | Forward primer | Reverse primer |
|---|---|---|
| RT-qPCR primers for genes with switched APA sites | ||
| COL1A2-S | ATCTACTTGCTTAAATTGTGGGC | GGTTGACATTTTCCATAACAGGT |
| COL1A2-L | GCCAGTCTCATTTTCATCTTCTT | ATGCTTTATTTCATTTTTTTCACAA |
| DDX5-S | ACATAAAGCAAGTGAGCGACC | CCTCTACCCCTGGAACGAC |
| DDX5-L | CTTTCGGGGGAGAGGGTA | CAGGCTGGACACAACACACAT |
| SNRNP200-S | TTTTGGGTAAAGGAGAGTTGAGC | AAGGGAAAGGAAGTGGAGGTAG |
| SNRNP200-L | ACTACCACAAGAACCAACACTGAG | GGGTCACATCCAGCTAGTACATTT |
| CTNNBIP1-S | ACTCAGTGGGGCTGGCAT | AAGGTTTCTGTTGGTCAAGATTTA |
| CTNNBIP1-L | GCCCCCTCTTTGTAGCTCCT | CAGCAACACTTTGACTTTTCCTCT |
| PPM1A-S | TGTGTTTGGACTTGGGGTT | AGTTAAATGAAGGGACTGGCT |
| PPM1A-L | CAACCACCACCAATGCACA | TAGTCAAGGGATAACCAGGTAAGA |
| DVL3-S | TGTGGATGTGATGTGAGCAGG | GACAAAGTAAAAAAGACGGACGG |
| DVL3-L | GTAGTCGCCTCCAATAGCCAT | GGTTAGTAGGGTTAGGGGTCTGAA |
| RT-qPCR primers for differentially expressed genes | ||
| MGP | CCTTCATATCCCCTCAGCAGA | GCAGCATTGTATCCATAAACCA |
| TGFBR3 | CCTTGGGGACAGTAGTGGTTG | GTGATGTTTCCGTGGGGCT |
| IGF2 | CATCGTTGAGGAGTGCTGTTTC | ACTGCTTCCAGGTGTCATATTG |
| RCAN1 | GATGCGACCCCAGTCATAAAC | TTCCTCTTCTTCCTCCTTCTCT |
| CCND1 | GCATCTACACCGACAACTCCA | TTGTTCTCCTCCGCCTCTG |
S, primer for shortened region of 3′UTRs; L, primer for lengthened region of 3′UTRs; APA, alternative polyadenylation.