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(A) The SERS electropherogram of the two sulfur-containing side chain amino acids indicates that Met migrates at tm = 216 ± 8 s and Cys at tm = 329 ± 6 s. The signal threshold (>4σ) was set to 680 counts. (B) Averaged SERS spectra of (i) Met and (ii) Cys were extracted from the electropherogram in (A) between 215.6 and 216.1 s, and 328.7 and 329.4 s, respectively. The amino acid concentration in this mixture is 5.0×10−4 M.
