Extended Data Figure 3. Reads mapping, exon coverage and missense somatic variants in RNA-Seq data.

a, Summary of total RNA-Seq read counts and mapping results for individual samples. b, Distribution of percentage sequence coverage in exons for individual samples. Among all 228,157 exons, 76% were expressed, but only 64% had an average coverage greater than 1. Exons with no coverage were not included in the box plots. c, Number of missense somatic variants detected by RNA-Seq in individual samples. Approximately 54% of the mutation positions were covered by RNA-Seq reads and only 43% were covered by three or more reads.