Fig. 3.

Examples of FaceCon modules and their effects on a strain׳s feasible region. The effects of the coupling (A), chemical level (B) and direct topology (C) modules are shown to illustrate each module׳s intent and capabilities. Here the orange dot indicates the optimal solution that would be found from including each module in OptORF. The dashed lines and hatched regions indicate the excluded regions imposed by the FaceCon modules.