Fig. 5.

Application of FaceCon to iJO1366. Parameters used in FaceCon modules for each case are provided above the mutant feasible regions. Dashed lines and hatched regions indicate excluded regions imposed by the FaceCon modules. The module included in OptORF for each case was (A) no FaceCon modules, (B) coupling module (only directional coupling), (C) coupling module (only weak coupling), and (D) direct topology module. OptORF without any modules (A) finds a lower chemical production phenotype compared to (B) and (C) due to gene deletion penalties used in OptORF. Additionally, no tilt was applied to the OptORF algorithm resulting in a strain design where there is no coupling between biomass and ethanol production. Alternate solutions exist, and replacing the glcA and lldP deletions with dld and ldhA deletions has a negligible effect on the feasible regions shown in panels (B), (C), and (D).