Figure 5. Recall proliferation and cytokine responses of lymphocytes from α-NETA-treated mice and induction of EAE by adoptive transfer of MOG-reactive lymphocytes.

MOG_35–55/CFA-immunized mice received α-NETA or captisol vehicle once daily. Ten days after immunization, spleen cells and draining LN cells were harvested and re-stimulated in vitro with the indicated concentrations of MOG_35–55. After 72 h of stimulation, (A) proliferation and (B) cytokine production by spleen cells (left panel) or LN cells (right panel) were evaluated. Proliferation data are presented as mean cpm ± SEM (triplicate wells); ELISA data are presented as mean cytokine concentration ± SEM (triplicate wells). (C) EAE was induced in C57BL/6 mice by passive transfer of MOG_35–55-reactive lymphocytes derived from vehicle-treated (n = 4) or α-NETA-treated mice (n = 7) as described in the Materials and Methods. The pooled data from two independent experiments is displayed, mean clinical score + SEM.