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. 2014 Aug 12;13:95. doi: 10.1186/s12934-014-0095-2

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© Nielsen et al. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Activity of different DmaW and EasF enzymes. Relative production of DMAT (black bars) and Me-DMAT (grey bars) were analysed in strains co-expressing EasF_Aj in combination with DmaW_Aj1 (A), DmaW_Aj2 (B), DmaW_Aj3 (C), DmaW_Af (D), or DmaW_Cp (E). Expression of DmaW_Aj1 and DmaW_Af resulted in only small amounts of compounds compared to the other three DmaW homologues. Similarly, DmaW_Aj2 was co-expressed with the two homologues EasF_Aj (G) or EasF_Af (H) and relative DMAT and Me-DMAT amounts were measured. The EasF_Af resulted in more than double the amount of Me-DMAT compared to EasF_Aj. The control strain (F) carried an empty plasmid with no DmaW expression. The vertical axis shows arbitrary units based on area under the HPLC peak.