Table 1. Sequences of primers used for Phusion-PCR.
primer | sequence (5′-3′) | length in bp |
PP30_75_4488F | tag aaa aaa cac ggg tag aag agt ctg gat ccc ggc tag cac att c | 434 |
PP30_75_4921R | gcc aca ccg cca ata atg gcg cct ata aag cgc ttc ttc ctc ctt act cc | |
PP30_75_4488F | tag aaa aaa cac ggg tag aag agt ctg gat ccc ggc tag cac att c | 1654 |
PP30_75_6141R | tgt tgc gcc tttt gct tgt aca tca ggt agc atg cta gaa c | |
PP30_75_4857F | ctg tga cta cat ctg gag gga gga gga aga agc gct tta tag gtg cc | 1451 |
PP30_75_6307R | gtc atc tac aac cgg tag ttt ttt ctt aaa tct tcc att gaa cag gtt gtc ag |