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. 2014 Dec 1;9(12):e113604. doi: 10.1371/journal.pone.0113604

Figure 1. Cryptoporus volvatus extract inhibits influenza virus infection in vitro.

Figure 1

(A and B) The Cryptoporus volvatus extract represses H1N1/BJ09 replication in MDCK cells. MDCK cells were infected with H1N1/BJ09 at an MOI of 0.1, and then treated with the Cryptoporus volvatus extract at various concentrations or the control normal saline. At 24 h p.i., cells were fixed and analyzed by IFA using antibody against virus NP protein (A), and virus yield in the supernatants was also quantified (B). Cultures treated with normal saline were set up as control (0 mg/ml). (C) Cryptoporus volvatus extract potently inhibits both A/Jiangxi (H3N2) and A/WSN (H1N1) replication in MDCK cells. A similar virus inhibition assay was performed with MDCK cells infected with A/Jiangxi (H3N2) or A/WSN (H1N1) at an MOI of 0.1 in the presence of the Cryptoporus volvatus extract at various concentrations or the control normal saline. (D) Determination of cytotoxicity of the Cryptoporus volvatus extract by MTT assay. MDCK cells were incubated with various concentrations of the Cryptoporus volvatus extract or the control normal saline for 48 h prior to the MTT assay. Data are representative of three independent experiments (mean ±SEM). Statistical significance was analyzed by One-way ANOVA. *P<0.05; **P<0.01.