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. 2014 Dec 1;9(12):e113775. doi: 10.1371/journal.pone.0113775

Figure 1. TF screening on the Nkx2.5 CE element by luciferase reporter assays.

Figure 1

A. Plasmid constructs for luciferase reporter assays. The empty modified pcDNA3.1 was used as a negative control in all assays. B. TF screening by luciferase assays using HEK 293 cells (human embryonic kidney fibroblasts). Fold change is compared to the negative control (neg ctr) (pcDNA3.1). C. TF screening by luciferase assays using H9c2 cells (rat myoblasts). Fold change is compared to the negative control (neg ctr) (pcDNA3.1). D. Mzf1 activates the Nkx2.5 CE element in atrial HL-1 cells but not in endothelial NFPE cells. Asterisks indicate a significant difference compared to the control (pcDNA3.1); **  =  p <0.01. E. Dose dependent effect of Mzf1-pcDNA3.1-DNA on Nkx2.5 CE activation in HEK 293 cells; *  =  p <0.05. F. Effect of truncating different parts of the Nkx2.5 CE according to Lien and co-workers [7] on luciferase activation by Mzf1 in HEK 293 cells.