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. 2014 Dec 1;9(12):e113629. doi: 10.1371/journal.pone.0113629

Table 3. Assessment of population nAb detection using reassortant pp.

Hemagglutination inhibitiona CPE- and fluorescence-based microneutralizationb
H5N1pp H5N1(09)pp H5N1pp H5N1(09)pp
270 samples collected in 2006
Positive rate 5/270 5/270 5/270 5/270
GMT (95% CI) 19.36 (7.89, 61.35) 18.35 (8.01, 55.38) 19.14 (8.91, 118.25) 16.57 (8.11, 109.63)
P 0.698 0.657
R2 1.00 1.00
H1N1pp H1N1(H5)pp H1N1pp H1N1(H5)pp
270 samples collected in 2006
Positive rate 68/270 68/270 68/270 68/270
GMT (95% CI) 30.54 (7.86, 234.41) 41.35 (8.96, 214.58) 59.32 (15.56, 236.58) 58.54 (18.32, 214.65)
P 0.678 0.576
R2 1.00 1.00
a

For the hemagglutination inhibition test, the titers of the virus and pp were adjusted to 4 hemagglutination units.

b

For the microneutralization assays, the titers of the virus and pp were adjusted to 100 TCID50. The 95% inhibitory concentration (IC95) was used for nAb analysis.