Table 3. Assessment of population nAb detection using reassortant pp.
Hemagglutination inhibitiona | CPE- and fluorescence-based microneutralizationb | |||
H5N1pp | H5N1(09)pp | H5N1pp | H5N1(09)pp | |
270 samples collected in 2006 | ||||
Positive rate | 5/270 | 5/270 | 5/270 | 5/270 |
GMT (95% CI) | 19.36 (7.89, 61.35) | 18.35 (8.01, 55.38) | 19.14 (8.91, 118.25) | 16.57 (8.11, 109.63) |
P | 0.698 | 0.657 | ||
R2 | 1.00 | 1.00 | ||
H1N1pp | H1N1(H5)pp | H1N1pp | H1N1(H5)pp | |
270 samples collected in 2006 | ||||
Positive rate | 68/270 | 68/270 | 68/270 | 68/270 |
GMT (95% CI) | 30.54 (7.86, 234.41) | 41.35 (8.96, 214.58) | 59.32 (15.56, 236.58) | 58.54 (18.32, 214.65) |
P | 0.678 | 0.576 | ||
R2 | 1.00 | 1.00 |
For the hemagglutination inhibition test, the titers of the virus and pp were adjusted to 4 hemagglutination units.
For the microneutralization assays, the titers of the virus and pp were adjusted to 100 TCID50. The 95% inhibitory concentration (IC95) was used for nAb analysis.