Figure 7. Fluorometric Ca²⁺ assay of thermoTRP channels to evaluate the inhibitory activity of compound 6a/b.

a) Activity of compound 6a/b at 50 µM on TRPA1, TRPV1 and TRPM8. b) Dose-response curve for TRPA1 inhibition by compound 6a/b. Solid lines depict the best fit to a binding isotherm, with IC₅₀  = 2.6±1.1 µM, and n_H (hill coefficient)  = 0.21. TRPA1 was activated with 100 µM AITC, TRPV1 was activated by 10 µM capsaicin and TRPM8 was activated with 100 µM menthol. Data are given as mean ± SEM, with n (number of experiments) ≥5.