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. 2014 Dec 1;9(12):e113875. doi: 10.1371/journal.pone.0113875

Figure 5. The transient expression of AvrRxo1 mutant proteins in Nicotiana benthamiana.

Figure 5

The expressions of avrRxo1RS105 (1), pGR106 (2) in N. benthamiana cells were used as positive and negative controls, respectively. Similarly hereinafter. (A) The C-terminus-truncated proteins AvrRxo1(1–159) (3), AvrRxo1(1–193) (4), AvrRxo1(1–278) (5), AvrRxo1(1–373) (6), AvrRxo1(1–412) (7) and AvrRxo1(1–420) (8) expressed in N. benthamiana all failed to induce cell death. (B) The transient expression of N-terminus truncated proteins in N. benthamiana. The expressions of AvrRxo1(52–421) (3) can still induce cell death, whereas AvrRxo1(109–421) (4) and AvrRxo1(140–421) (5) loss the toxic ability in N. benthamiana. (C) The transient expression of mutant proteins in N. benthamiana. Transient expression of avrRxo1(G165A) (3), avrRxo1(K166N) (4), and avrRxo1(H71A) (6) did not induce cell death, however expression of avrRxo1(nls) (5) still resulted in cell death in N. benthamiana. The normal expression of avrRxo1 in infected leaves were confirmed by RT-PCR. All experiments were repeated three times with similar results.