Figure 7. Quantification of vMOs knock-down effect on three aspects of skeleton growth in juvenile rudiments.

See Figures 5 and 6 for details on which skeletal elements were scored. A) qRT-PCR results for p58b and p16 in three development stages show expression of these genes in juvenile stages. B) spine elongation, measured by the number of cross hatches in adult type spines over a time period 96 h post-injection. C) number of adult type pre-spines present in the oral region of the juvenile rudiment 96 h post-injection D) number of adult type spines present in the oral region of the juvenile rudiment 96 h post-injection. Lines above bars indicate significant differences (p<0.05) between pairs of treatments.