Figure 4. Microvascular structure and neuroblasts in the V/SVZ.

Double immunofluorescent images acquired from representative non-ischemic (A) and ischemic (B) whole mounts (viewed from the ventricular surface) show the network of collagen IV⁺ blood vessels (red) and distribution of GFP⁺ neuroblasts (green). Projected images with total thickness of 50 µm from the Z axis (C, D) show that GFP⁺ neuroblasts in non-ischemic whole mount were restricted in the SVZ (C, green), while GFP⁺ neuroblasts in ischemic whole mount distributed from the SVZ (D, green) down to striatum (D, green). Single composite image with 1 µm thickness from X–Y axis at SVZ and striatum beneath the ventricular surface from their corresponding Z stacks (C, D) show collagen IV⁺ blood vessels and GFP⁺ neuroblasts.