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. 2014 Dec 1;9(12):e114117. doi: 10.1371/journal.pone.0114117

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© 2014 Cao et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Panel A: Immunoblot of eEF1Bα, eEF1Bδ and eEF1Bγ protein expression in cell lines. GAPDH was used as a loading control. Panel B: Immunoblot of eEF1B subunits in 24-day old mice. Each tissue is shown twice, the left hand sample is from a wild type mouse and the right hand sample from a wasted homozygous mouse. SC = spinal cord. The second row shows a longer exposure of the eEF1Bδ blot, revealing the expression of the longer isoform. GAPDH was used as a loading control. Panel C: Immunoblot of eEF1Bδ in an extended panel of mouse tissues showing brain and testis-expression of the longer isoform (top panel shows a longer exposure). GAPDH was used as a loading control. Panel D: Immunoblot of eEF1B subunits expression in brain (Br) and liver (Li) throughout late embryonic and postnatal mouse development. GAPDH was used as a loading control.