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. 2014 Nov 10;111(47):16760–16765. doi: 10.1073/pnas.1419240111

Fig. 1.

Fig. 1.

4C-Seq analysis reveals the physical association of the ANO1 gene locus with the INS promoter in human pancreatic islets. (A) 4C-Seq analysis of INS-associated loci in the IDDM4 locus in chromosome 11 (NCBI36/hg18) (Upper) and the ANO1 gene locus within the IDDM4 (Lower). The four Bgl II sites used in 3C-PCR for confirmation of 4C results are shown. (B) 3C-PCR analysis of the interactions of the INS promoter with the ANO1/FADD genes and the nearby FGF3 and PPF1A1 genes in human islets cultured in the basal islet media containing 5.5 mM glucose. (C) TaqMan quantitative 3C analysis of the INS–ANO1 interactions in islets, islet-derived hIPCs, and primary human fibroblasts. Data are mean ± SEM (n = 8). (D) TaqMan quantitative 3C analysis of the INS–ANO1 interactions in islets from two donors before and after treatment with 25 mM glucose for 1 h. Data are mean ± SEM (n = 8). (E) qRT-PCR analysis of ANO1 (orange bar) and FADD (green bar) gene expression in islets from three donors before and after treatment with 25 mM glucose for the indicated times. The RNA levels are normalized to those of HPRT1. Plotted are mRNA levels relative to those at t = 0. Data are mean ± SEM (n = 8).