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. 2014 Nov 10;111(47):16760–16765. doi: 10.1073/pnas.1419240111

Fig. 2.

Fig. 2.

The INS promoter positively regulates ANO1 gene expression in human islets. (A) qRT-PCR analysis of INS (red bar), ANO1 (orange bar), and FADD (black bar) gene expression in islets and islet-derived hIPCs from two donors. Plotted are mRNA levels in hIPCs relative to those in the corresponding islets. Data are mean ± SEM (n = 8). (B) qRT-PCR analysis of ANO1 (orange bar) and FADD (black bar) gene expression in islets from two donors treated with nontargeting control (Ctl) siRNA or one of the two siRNAs targeting to the INS promoter (INSP). The mRNA levels are plotted relative to the nontargeting control. (C) Promoter luciferase reporter assays in transfected MIN6 mouse β cells. The human ANO1 promoter luciferase constructs without (Top) or with insertion of human INS promoter in the forward (Middle) or reverse (Bottom) orientation was transfected into MIN6 cells. Plotted are the reporter luciferase activities relative to the ANO1 promoter luciferase construct without the INS promoter. Data are mean ± SEM (n = 12).