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. 2014 Nov 10;111(47):16814–16819. doi: 10.1073/pnas.1414189111

Fig. 4.

Fig. 4.

KD025 promotes the suppressive function of Tregs via up-regulation of STAT5 phosphorylation and Foxp3 expression. CD4+ T cells were treated with indicated doses of KD025 and then stimulated by anti-CD3/28 mAbs, IL-1β, and TGF-β for 48 h (A and B) or 120 h (D). Whole cellular extracts were prepared and analyzed by Western blot (A–C). Foxp3 expression was tested after 120 h (D). The average of three different experiments is shown. (E) Freshly purified human CD4+CD25hiCD127lo (Tregs) were treated with KD025, mixed with labeled PBMCs (ratio 1:1), and cultured in the presence of soluble anti-CD3 antibodies for 96 h. Cell proliferation was determined by CFSE dilution (E). The average of seven different experiments is shown. STAT5 phosphorylation was evaluated in lysates from purified Tregs treated with KD025 and then activated by anti-CD3/28 mAbs. *P < 0.05.