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. 2014 Nov 10;111(47):16814–16819. doi: 10.1073/pnas.1414189111

Fig. 5.

Fig. 5.

Treatment with KD025 down-regulates proinflammatory cytokine secretion in CD4+ T cells purified from RA patients and stops the progression of CIA in mice. Peripheral blood CD4+ T cells from RA patients were treated with 10 μM or indicated doses of KD25 for 1 h and then stimulated by immobilized anti-CD3/28 mAbs alone (A–C) or in combination with IL-1β and TGF-β (D and E). Cytokine secretion was determined after 48 h (A and B), and whole cell lysates were analyzed by Western blot (C). The average of four healthy and 11 different RA patients (A and B) and representative of five (C) different experiments is shown. The P value was calculated by t test. (D) Clinical arthritis scores of CIA mice treated with vehicle or indicated doses of KD025 (n = 10 per group). The treatment began on day 25 after immunization with collagen. Data are presented as the mean clinical score ± SEM. Representative H&E-stained sections in CIA mice killed at day 28 of the treatment. (E) Flow cytometry analysis of spleenocytes from CIA mice (day 28); data are shown as average percentage (n = 10) ± SD of total CD4+ T cells. Levels of IRF4, pSTAT3, and pSTAT5 were determined by Western blot (F). One representative of three different Western blots is shown.