Skip to main content
. 2014 Nov 10;111(47):16848–16853. doi: 10.1073/pnas.1404047111

Fig. 3.

Fig. 3.

Integration of dif1 and TLCϕ TS plasmids harboring C* and D* mutated overlap regions. (A) Scheme of the WT, C*, and D* overlap regions. Mutations are indicated in red. Positions of XerC (●) and XerD (○) cleavage are indicated. (B) Frequency of integrants after overnight growth at the permissive temperature obtained with WT, C*, and D* versions of dif1 and attPTLC. The scheme legend is as in Fig. 2B. (C) Frequency of integrants after 3 h of growth at the permissive temperature in XerC-inducible cells. n.d., none detected; off, no XerC induction; on, XerC induction; ParaXerC, production of XerC from under the pAra promoter integrated at the xerC locus. The rest of the legend is as in Fig. 2B. (D) Frequency of integrants after 3 h of growth at the permissive temperature in ParaXerC ftsKΔγ cells. The legend is as in C.