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. 2014 Dec 1;9(12):e114029. doi: 10.1371/journal.pone.0114029

Figure 3. Reduction in the calcium-binding affinity of holo-LA in the presence of Ficoll 70.

Figure 3

(A) Far-UV CD spectra of holo-LA at 45°C under different solvent conditions: dilute (solid line); 1.2 mM EDTA (dashed line) and 300 g/l Ficoll 70 (dotted line). (B) Near-UV CD spectra of holo-LA at 45°C under different solvent conditions: dilute (solid line); 1.2 mM EDTA (dashed line) and 300 g/l Ficoll 70 (dotted line). (C) Representative thermal denaturation profiles of holo-LA in the presence of varying calcium chloride concentrations under dilute (solid symbols) and crowded (open symbol) conditions. In order to maintain clarity, some transition curves have not been shown.