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. 2014 Dec 1;9(12):e114029. doi: 10.1371/journal.pone.0114029

Figure 4. Calcium inhibits crowding-induced holo-LA aggregation.

Figure 4

(A) Plot of ANS fluorescence intensity (at 473 nm) versus [CaCl2] (solid circle) and percent soluble protein versus [CaCl2] (solid triangle) under Ficoll 70 treated conditions. (B) Electron micrograph of holo-LA following heat-induced denaturation in the presence of 400 g/l Ficoll 70 and 100 mM calcium chloride. (C) Far-UV CD spectra of holo-LA at 45°C under different solvent conditions: dilute (solid line); 300 g/l Ficoll 70 (dashed line) and 300 g/l Ficoll 70 plus 100 mM CaCl2 (dotted line). (D) Near-UV CD spectra of holo-LA at 45°C under solvent conditions: dilute (solid line); 300 g/l Ficoll 70 (dashed line) and 300 g/l Ficoll 70 plus 100 mM CaCl2 (dotted line).